The hypocotyl region of an Arabidopsis thaliana (Mouse-ear Cress) plant that expresses a membrane protein CPK30GFP (Green Fluorescent Protein fused to the Calcium Dependent Protien Kinase 30) was used to take a time lapse image of vesicle trafficking in action. The vesicle trafficking was stimulated by auxin (NAA) and captured using a fluorescent microscope. The red dots in the picture are the result of chloroplasts giving off a signal (auto-fluorescence) when subjected to UV light. The white lines, which represent the GFP signal, are a result of the red dots. The green line points out a large moving section that takes up a smaller vesicle. The blue line points to a vesicle moving in the three-dimensional space, causing it to come in and out of focus as it passes the two-dimensional plane. The yellow line points out a cluster of vesicles moving as a larger one passes.